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KMID : 0848120080330030091
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International Journal of Oral Biology
2008 Volume.33 No. 3 p.91 ~ p.95
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Osteogenic Differentiation Potential in Parthenogenetic Murine Embryonic Stem Cells
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Kang Ho-In
Cha Eun-Sook
Choi Young-Ju
Min Byung-Moo
Roh Sang-Ho
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Abstract
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Embryonic stem cells have a pluripotency and a potential to differentiate to all type of cells. In our previous study, we have shown that embryonic stem cells (ESCs) lines can be generated from murine parthenogenetic embryos. This parthenogenetic ESCs line can be a useful stem cell source for tissue repair and regeneration. The defect in full-term development of parthenogenetic ESCs line enables researchers to avoid the ethical concerns related with ESCs research. In this study, we presented the results demonstrating that parthenogenetic ESCs can be induced into osteogenic cells by supplementing culture media with ascorbic acid and β-glycerophosphate. These cells showed morphologies of osteogenic cells and it was proven by Von Kossa staining and Alizarin Red staining. Expression of marker genes for osteogenic cells (osteopontin, osteonectin, alkaline phosphatase, osteocalcin, bone-sialoprotein, collagen type1, and Cbfa1) also confirmed osteogenic potential of these cells. These results demonstrate that osteogenic cells can be generated from parthenogenetic ESCs in vitro.
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KEYWORD
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Embryonic stem cell, Parthenogenesis, Osteogenic cell
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